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Promega ratp standard
Ratp Standard, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ratp+standard/ratp+standard/pmc11402965-291-16-18
Average 90 stars, based on 1 article reviews
ratp standard - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Normothermic ex vivo kidney perfusion preserves mitochondrial and graft function after warm ischemia and is further enhanced by AP39.
Article Snippet: Intracellular ATP levels in biopsy-derived kidney cells were measured using the CellTiter-Glo® 2.0 Assay kit and rATP standard (Promega,WI, USA).

Membrane:

Article Title: The effect of different carbon sources on biofouling in membrane fouling simulators: microbial community and implications.
Article Snippet: .. The RLU measurements were converted to nmol L 1 using a Promega rATP standard, and the result was converted to pg cm 2 ATP using the volume of water added and the membrane surface area. ..

Affinity Purification:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process
Article Snippet: .. Antibodies and Reagents—Antibodies were obtained as follows: mouse anti-CR1 mIgG1 YZ1 (11) and 3C10 (gifts of Dr. Lloyd Klickstein, formerly Harvard Medical School, now Novartis); affinity-purified custom made extra- and intracellular chicken anti-human pannexin-1 pIgY (4512, 4515) (gift of Dr. Gerhard Dahl, University of Miami, Miami, FL, and Dr. Eliana Scemes, Albert Einstein College ofMedicine, New York) (12); AffiniPure goat anti-mouse IgG and ChromPure mouse IgG (115-005-003 and 115-001-003, Jackson ImmunoResearch, West Grove, PA); mouse anti-CD47 (556044, BD Biosciences, Franklin Lakes, NJ); LEAF purified mouse IgG1 (401404, Biolegend, San Diego, CA); Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-chicken IgG, Alexa Fluor 488 F(ab )2 fragment of goat anti-rabbit IgG, Alexa Fluor 594 goat anti-mouse IgG, rabbit anti-BSA pAb, rabbit anti-pannexin 1 pAb (N-term), Zenon Alexa Fluor 488 mouse IgG1 labeling kit, (A11029, A11039, A11070, A11032, A11133, 487900, and Z25002, Invitrogen); 2-2Zn(II) probe (Dr. Itaru Hamachi, KyotoUniversity, Japan) (13); KT5720, 4,5,6,7-tetrabromobenzotriazole (TBB), 10Panx, scrambled 10Panx, ARL 67156 (all R&D Systems, Minneapolis, MN); apyrase from potato with 50% of base activity ADPase (A6535), MK-571, 5 mM EGTA supplemented with 5 mM Mg2 (designated Mg2 -EGTA in text), citrate (4%w/v), dextranT500, gelatin veronal buffer (GVB), ovalbumin (A5503), zymosan (all from Sigma); IgG and protease- free BSA (001-000-162, Jackson ImmunoResearch); DiO, Hanks’ balanced salt solutionwith (HBSS2 ) andwithout (HBSS ) CaCl2 andMgCl2, 1 mFluoSpheres ( excitation/emission 505/515) and 0.2- m non-fluorescent sulfate latex microspheres (microbeads), bovine serum albumin (BSA) Alexa Fluor 647 conjugate (D-275, 14025, 14175, F-8852, S37491, A34785, Invitrogen); ENLITEN rLuciferase/Luciferin ATP assay reagent and 10 mM rATP standard (FF2021, P1132, Promega, Madison, WI); Ficoll and Percoll (GE Healthcare, Pittsburgh, PA); andHEK293T cells (gift ofDr.Gina Sosinsky,University of California, San Diego). ..

Purification:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process
Article Snippet: .. Antibodies and Reagents—Antibodies were obtained as follows: mouse anti-CR1 mIgG1 YZ1 (11) and 3C10 (gifts of Dr. Lloyd Klickstein, formerly Harvard Medical School, now Novartis); affinity-purified custom made extra- and intracellular chicken anti-human pannexin-1 pIgY (4512, 4515) (gift of Dr. Gerhard Dahl, University of Miami, Miami, FL, and Dr. Eliana Scemes, Albert Einstein College ofMedicine, New York) (12); AffiniPure goat anti-mouse IgG and ChromPure mouse IgG (115-005-003 and 115-001-003, Jackson ImmunoResearch, West Grove, PA); mouse anti-CD47 (556044, BD Biosciences, Franklin Lakes, NJ); LEAF purified mouse IgG1 (401404, Biolegend, San Diego, CA); Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-chicken IgG, Alexa Fluor 488 F(ab )2 fragment of goat anti-rabbit IgG, Alexa Fluor 594 goat anti-mouse IgG, rabbit anti-BSA pAb, rabbit anti-pannexin 1 pAb (N-term), Zenon Alexa Fluor 488 mouse IgG1 labeling kit, (A11029, A11039, A11070, A11032, A11133, 487900, and Z25002, Invitrogen); 2-2Zn(II) probe (Dr. Itaru Hamachi, KyotoUniversity, Japan) (13); KT5720, 4,5,6,7-tetrabromobenzotriazole (TBB), 10Panx, scrambled 10Panx, ARL 67156 (all R&D Systems, Minneapolis, MN); apyrase from potato with 50% of base activity ADPase (A6535), MK-571, 5 mM EGTA supplemented with 5 mM Mg2 (designated Mg2 -EGTA in text), citrate (4%w/v), dextranT500, gelatin veronal buffer (GVB), ovalbumin (A5503), zymosan (all from Sigma); IgG and protease- free BSA (001-000-162, Jackson ImmunoResearch); DiO, Hanks’ balanced salt solutionwith (HBSS2 ) andwithout (HBSS ) CaCl2 andMgCl2, 1 mFluoSpheres ( excitation/emission 505/515) and 0.2- m non-fluorescent sulfate latex microspheres (microbeads), bovine serum albumin (BSA) Alexa Fluor 647 conjugate (D-275, 14025, 14175, F-8852, S37491, A34785, Invitrogen); ENLITEN rLuciferase/Luciferin ATP assay reagent and 10 mM rATP standard (FF2021, P1132, Promega, Madison, WI); Ficoll and Percoll (GE Healthcare, Pittsburgh, PA); andHEK293T cells (gift ofDr.Gina Sosinsky,University of California, San Diego). ..

Labeling:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process
Article Snippet: .. Antibodies and Reagents—Antibodies were obtained as follows: mouse anti-CR1 mIgG1 YZ1 (11) and 3C10 (gifts of Dr. Lloyd Klickstein, formerly Harvard Medical School, now Novartis); affinity-purified custom made extra- and intracellular chicken anti-human pannexin-1 pIgY (4512, 4515) (gift of Dr. Gerhard Dahl, University of Miami, Miami, FL, and Dr. Eliana Scemes, Albert Einstein College ofMedicine, New York) (12); AffiniPure goat anti-mouse IgG and ChromPure mouse IgG (115-005-003 and 115-001-003, Jackson ImmunoResearch, West Grove, PA); mouse anti-CD47 (556044, BD Biosciences, Franklin Lakes, NJ); LEAF purified mouse IgG1 (401404, Biolegend, San Diego, CA); Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-chicken IgG, Alexa Fluor 488 F(ab )2 fragment of goat anti-rabbit IgG, Alexa Fluor 594 goat anti-mouse IgG, rabbit anti-BSA pAb, rabbit anti-pannexin 1 pAb (N-term), Zenon Alexa Fluor 488 mouse IgG1 labeling kit, (A11029, A11039, A11070, A11032, A11133, 487900, and Z25002, Invitrogen); 2-2Zn(II) probe (Dr. Itaru Hamachi, KyotoUniversity, Japan) (13); KT5720, 4,5,6,7-tetrabromobenzotriazole (TBB), 10Panx, scrambled 10Panx, ARL 67156 (all R&D Systems, Minneapolis, MN); apyrase from potato with 50% of base activity ADPase (A6535), MK-571, 5 mM EGTA supplemented with 5 mM Mg2 (designated Mg2 -EGTA in text), citrate (4%w/v), dextranT500, gelatin veronal buffer (GVB), ovalbumin (A5503), zymosan (all from Sigma); IgG and protease- free BSA (001-000-162, Jackson ImmunoResearch); DiO, Hanks’ balanced salt solutionwith (HBSS2 ) andwithout (HBSS ) CaCl2 andMgCl2, 1 mFluoSpheres ( excitation/emission 505/515) and 0.2- m non-fluorescent sulfate latex microspheres (microbeads), bovine serum albumin (BSA) Alexa Fluor 647 conjugate (D-275, 14025, 14175, F-8852, S37491, A34785, Invitrogen); ENLITEN rLuciferase/Luciferin ATP assay reagent and 10 mM rATP standard (FF2021, P1132, Promega, Madison, WI); Ficoll and Percoll (GE Healthcare, Pittsburgh, PA); andHEK293T cells (gift ofDr.Gina Sosinsky,University of California, San Diego). ..

Activity Assay:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process
Article Snippet: .. Antibodies and Reagents—Antibodies were obtained as follows: mouse anti-CR1 mIgG1 YZ1 (11) and 3C10 (gifts of Dr. Lloyd Klickstein, formerly Harvard Medical School, now Novartis); affinity-purified custom made extra- and intracellular chicken anti-human pannexin-1 pIgY (4512, 4515) (gift of Dr. Gerhard Dahl, University of Miami, Miami, FL, and Dr. Eliana Scemes, Albert Einstein College ofMedicine, New York) (12); AffiniPure goat anti-mouse IgG and ChromPure mouse IgG (115-005-003 and 115-001-003, Jackson ImmunoResearch, West Grove, PA); mouse anti-CD47 (556044, BD Biosciences, Franklin Lakes, NJ); LEAF purified mouse IgG1 (401404, Biolegend, San Diego, CA); Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-chicken IgG, Alexa Fluor 488 F(ab )2 fragment of goat anti-rabbit IgG, Alexa Fluor 594 goat anti-mouse IgG, rabbit anti-BSA pAb, rabbit anti-pannexin 1 pAb (N-term), Zenon Alexa Fluor 488 mouse IgG1 labeling kit, (A11029, A11039, A11070, A11032, A11133, 487900, and Z25002, Invitrogen); 2-2Zn(II) probe (Dr. Itaru Hamachi, KyotoUniversity, Japan) (13); KT5720, 4,5,6,7-tetrabromobenzotriazole (TBB), 10Panx, scrambled 10Panx, ARL 67156 (all R&D Systems, Minneapolis, MN); apyrase from potato with 50% of base activity ADPase (A6535), MK-571, 5 mM EGTA supplemented with 5 mM Mg2 (designated Mg2 -EGTA in text), citrate (4%w/v), dextranT500, gelatin veronal buffer (GVB), ovalbumin (A5503), zymosan (all from Sigma); IgG and protease- free BSA (001-000-162, Jackson ImmunoResearch); DiO, Hanks’ balanced salt solutionwith (HBSS2 ) andwithout (HBSS ) CaCl2 andMgCl2, 1 mFluoSpheres ( excitation/emission 505/515) and 0.2- m non-fluorescent sulfate latex microspheres (microbeads), bovine serum albumin (BSA) Alexa Fluor 647 conjugate (D-275, 14025, 14175, F-8852, S37491, A34785, Invitrogen); ENLITEN rLuciferase/Luciferin ATP assay reagent and 10 mM rATP standard (FF2021, P1132, Promega, Madison, WI); Ficoll and Percoll (GE Healthcare, Pittsburgh, PA); andHEK293T cells (gift ofDr.Gina Sosinsky,University of California, San Diego). ..

ATP Assay:

Article Title: CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process
Article Snippet: .. Antibodies and Reagents—Antibodies were obtained as follows: mouse anti-CR1 mIgG1 YZ1 (11) and 3C10 (gifts of Dr. Lloyd Klickstein, formerly Harvard Medical School, now Novartis); affinity-purified custom made extra- and intracellular chicken anti-human pannexin-1 pIgY (4512, 4515) (gift of Dr. Gerhard Dahl, University of Miami, Miami, FL, and Dr. Eliana Scemes, Albert Einstein College ofMedicine, New York) (12); AffiniPure goat anti-mouse IgG and ChromPure mouse IgG (115-005-003 and 115-001-003, Jackson ImmunoResearch, West Grove, PA); mouse anti-CD47 (556044, BD Biosciences, Franklin Lakes, NJ); LEAF purified mouse IgG1 (401404, Biolegend, San Diego, CA); Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-chicken IgG, Alexa Fluor 488 F(ab )2 fragment of goat anti-rabbit IgG, Alexa Fluor 594 goat anti-mouse IgG, rabbit anti-BSA pAb, rabbit anti-pannexin 1 pAb (N-term), Zenon Alexa Fluor 488 mouse IgG1 labeling kit, (A11029, A11039, A11070, A11032, A11133, 487900, and Z25002, Invitrogen); 2-2Zn(II) probe (Dr. Itaru Hamachi, KyotoUniversity, Japan) (13); KT5720, 4,5,6,7-tetrabromobenzotriazole (TBB), 10Panx, scrambled 10Panx, ARL 67156 (all R&D Systems, Minneapolis, MN); apyrase from potato with 50% of base activity ADPase (A6535), MK-571, 5 mM EGTA supplemented with 5 mM Mg2 (designated Mg2 -EGTA in text), citrate (4%w/v), dextranT500, gelatin veronal buffer (GVB), ovalbumin (A5503), zymosan (all from Sigma); IgG and protease- free BSA (001-000-162, Jackson ImmunoResearch); DiO, Hanks’ balanced salt solutionwith (HBSS2 ) andwithout (HBSS ) CaCl2 andMgCl2, 1 mFluoSpheres ( excitation/emission 505/515) and 0.2- m non-fluorescent sulfate latex microspheres (microbeads), bovine serum albumin (BSA) Alexa Fluor 647 conjugate (D-275, 14025, 14175, F-8852, S37491, A34785, Invitrogen); ENLITEN rLuciferase/Luciferin ATP assay reagent and 10 mM rATP standard (FF2021, P1132, Promega, Madison, WI); Ficoll and Percoll (GE Healthcare, Pittsburgh, PA); andHEK293T cells (gift ofDr.Gina Sosinsky,University of California, San Diego). ..



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